ABSTRACT
Objective To discuss the effect of Dickkopf-1 (DKK-1) on Wnt singal pathway during the differentiation of osteoblast in vitro.Methods The osteoblasts were obtained from the new rats and cultured in vitro.The 3 passages were divided into control group and DKK-1 group.The cells were cultured in DKK-1 and normal saline for morphogical detection,ALP activity detection and osteoblasts stained at 1 st,6th,12th,21st day.The Wnt was detected by RT-PCR.Results After cultured by the DKK-1 in vitro,the ALP and mineralization of osterblasts staining were prlonged with culture time.Compared with control group,the expression of Wnt was significantly reduced at the 21st day after induction (t =0.278,P ＜ 0.05).Conclusion DKK-1 can regulate the expression of Wnt during osteoblast differentiation,suggests that Wnt may be involved in osteoblast differentiation and can affect bone remodeling process.
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[Objective]To evaluate and compare the of the ideals surgical technique for intertrochanteric fractue between DHS and arthroplasty.[Method]A total of 40 gerontal patients with the different surgical treatment from January 1999 to May 2004 were chosen. The mean age of the patients was 82 (75～90).Among the patients, 23 patients by DHS,17 by arthroplasty.[Result]The duration of follow-up averaged 1.5 years.The time in bed were 3 and 1 weeks due to DHS and arthroplasty, the full weight-bearing time were 10 and 6 weeks.[Conclusion]arthroplasty is ideal choice for the un-stable intertrochanteric fractue.
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Objective: To investigate effect of Zhuanggu Granula medicated serum on proliferation and differentiation of bone marrow stromal cells(BMSCs) in vitro. Methods: Isolated BMSCs were cultured in vitro. The cells were treated with Zhuanggu Granula medicated serum, and the blank serum as the control group. The proliferation of cells was detected by MTT assay, the macroscopic histology, HE staining and immunohistoc-hemical examinations. Results: Zhuanggu Granula medicated serum effectively stimulated BMSCs proliferation (P